Development of a novel PCR assay specific for Riemerella anatipestifer
نویسندگان
چکیده
منابع مشابه
Development and application of specific polymerase chain reaction assay targeting the gyrB gene for rapid detection of Riemerella anatipestifer.
A pair of PCR primers was designed and synthesized to amplify a gyrB gene sequence from Riemerella anatipestifer (RA). A fragment of 194 bp was detected in RA-positive isolates, whereas other isolates were negative, which confirmed the high specificity of the primers and PCR conditions. As little as 1.6 × 10(4) cfu/mL of cultural liquid was required by this method. We compared a 16S rRNA sequen...
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Received: Revised: Accepted: August 15, 2010 August 31, 2010 September 10, 2010
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To establish an accurate, rapid, and a quantifiable method for the detection of Riemerella anatipestifer infection, a widespread infectious disease in birds, we developed a TaqMan-based real-time PCR assay by using DtxR gene-specific primers and a TaqMan probe. The standard curve established with a linear correlation (R2) of 0.998 and efficiency of 99% between the Ct value and the logarithm of ...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2007
ISSN: 0266-8254,1365-2673
DOI: 10.1111/j.1472-765x.2006.02053.x